pH
Each enzyme has an optimum pH, above or below which their activity is lower.
Supply students with 5 ph buffers i.e ph 2,4,7,10 and 12
Activity was measured by transmittance and absorbance using a Mystrica colourimeter
Temperature:
All reactions are faster at a higher temperature, however the enzyme-catalyzed reactions become slower or stop if the temperature is too low or too high, because enzymes become denatured at high temperature. Set up an ice bath, a room temperature water bath (about 22°C), a water bath at body temperature (37°C), one at 50°C and one at 75°C
The students add 2ml of enzyme and 2 ml of solution in different test tubes and left them at the different temperatures.
After 5 minutes they check for differences by using a Mysterica colourimeter