Enzymes- recipes:

Diastase:
Recommended working strength: 0.1 gram powder/100 ml 0.1% buffer
Reaction buffer: 0.9% Sodium chloride, 0.01% Calcium chloride, pH7
Optimum temperature: 20 -25 degrees C
Solution: always use fresh; will last for 12 hours at 4 degrees C
Pepsin:
Recommended strength: 0.1-0.5 gram/100 ml 0.1 -0.5% buffer
Reaction buffer: 0.9% Sodium chloride at pH2; acidify with glacial acetic acid added drop by drop
Optimum temperature: 35 -40 degrees C
Solution: fresh solution is best; will last for 18 hrs at 4 degrees C or 12 hrs at room temperature

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