Chlorophyll chromatography

Question:

What is the best solvent to use in the extraction of pigments from chlorophyll? We have tried ethanol, acetone and a 50/50 solution of the two, but the bands aren’t great.

Answer:

You can use 1:1 ethanol: petroleum ether to extract the pigments then 2 percent ethanol : 98 percent petroleum ether to separate them.

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